Category: General

Introduction to DNA Cloning

DNA cloning is one of the ways to create many identical copies of a piece of DNA, for example, a gene. In a classic cloning experiment, the target gene is inserted into a circular DNA molecule called a plasmid. The plasmid is introduced into bacteria through a process called transformation. Bacteria carrying the plasmid are…

Top 3 Experiments with DNA

Our modern idea of DNA and heredity has led to the development of a large number of trends, such as forensic analysis, paternity testing and genetic screening. Therefore, thanks to such widespread use, today many people have the basic idea of what DNA is. Proteins and DNA According to Gregor Mendel signs (such as painting…

Bioinformatics and Data Analysis

Bioinformatics is an interdisciplinary field that combines general biology, molecular biology, cybernetics, genetics, chemistry, computer science, mathematics and statistics. Large-scale biological problems requiring the analysis of large amounts of data are solved by bioinformatics from a computational point of view. Bioinformatics mainly includes the study and development of computer methods and is aimed at obtaining,…

DNA Microarray – Steps with Chip Experiments

DNA microchips are used to identify genes and their mutations associated with various diseases; monitoring of gene activity; diagnosis of infectious diseases and determining the most effective antibiotic therapy method; screening microorganisms. DNA microarrays, in contrast to real-time PCR technologies or mass spectrometry, allow you to analyze many mutations in one person at the same…

Affymetrix – Platform for DNA Microchips

The Affymetrix platform is a comprehensive solution for high-throughput genetic analysis based on the hybridization of fluorescently-labeled genome fragments with oligonucleotide DNA probes attached to a solid support (DNA microarray technology). These solutions are widely used for basic and applied research of DNA (for genotyping) and RNA (for analyzing gene expression). DNA chip format: cartridges,…

The Basics of DNA Extraction in the Laboratory

We will tell you how the first stage of genetic research is going on – the isolation of genomic DNA from biological samples. What is DNA? DNA is a long, negatively charged molecule: it codes for the assembly instructions for individual proteins, cells, tissues, and whole organisms. DNA is found in all cells with rare…

PCR

PCR is a laboratory diagnostic method aimed at identifying the causative agents of infectious diseases. The three-letter version is an abbreviation of the name “polymerase chain reaction”. Actually, this name reflects the essence of the method, but in order to understand it, you will have to thoroughly recall the school course in biology. But first,…

What is Methylation?

Methylation is a biochemical reaction for the attachment / detachment of the methyl group -CH3, which takes place in every cell of our body billions of times per second. For the optimal course of this process, a sufficient intake of amino acids (methionine), vitamins (B2, B6, B12, folates), minerals (zinc, iron), as well as choline…